Aqueous leaf extracts of Albizia lebbeck induce histological changes of reproductive organs of alloxan induced diabetic albino rats.

Volume 1, Issue 2, December 2016     |     PP. 336-353      |     PDF (1734 K)    |     Pub. Date: January 16, 2017
DOI:    362 Downloads     6938 Views  

Author(s)

Odey Daisy Mary, Department of Medical Laboratory Services University of Abuja Teaching Hospital PMB 228 Gwagwalada FCT Abuja, Nigeria.
Amos Dangana, Department of Medical Laboratory Services University of Abuja Teaching Hospital PMB 228 Gwagwalada FCT Abuja, Nigeria.
Idris Abdullah Nasir, Department of Medical Laboratory Services University of Abuja Teaching Hospital PMB 228 Gwagwalada FCT Abuja, Nigeria.
James Adisa, Department of Medical Laboratory Science Faculty of Medical Science, University of Jos

Abstract
AbstractBackground: The frequent use of herbal preparations for treatment of infections and diseases have led to the need to investigate possible side effects that could be associated with these plants. Objective of study: This study was designed to investigate the effects of aqueous leaf extracts of Albizzia lebbeck on the histology of the male and female reproductive organs of alloxan induced diabetic Albino rats.Materials and methods:The rats (n = 40), weighing 100 – 150g were randomly assigned into five treatments groups (A-E). The rats were induced with diabetes using Alloxan (100mg/kg) administered interperitoneally (Groups B, C, D). Treatment groups were treated with distilled water (Group A), and aqueous leaf extract of Albizzia lebbeck (100mg to group C and E, 200mg to group D) administered orally via orogastric tubes. Results: All the rats were fed with pellets and clean water ad libitum. Cage side examination was done daily to observe for behavioral signs. It was observed that Albizzia lebbeck leaf extract in female rats had no effect on the histology of the uterine endometrium, but, the ovaries were found to have a decreasing number of follicles indicating a possible arrest of oogenesis. The male rat’s testes showed a parallel decrease in germinal epithelium in all groups from group A to group E indicating a decrease in spermatogenesis. It was also noted that Albizzia lebbeck caused a decrease in feed intake and animal weight in all treatment groups during the course of this study. Conclusion:Findings from The aqueous extract of A. lebbeck leaves causes spermatogenic and oogenic arrest in male and female albino rats respectively. However, Albizzia lebbeck should be subjected tofurther analysis in order to unravel the active ingredients responsible for their antifertility actions.

Keywords
Antifertility; Albizzia lebbeck; Histology

Cite this paper
Odey Daisy Mary, Amos Dangana, Idris Abdullah Nasir, James Adisa, Aqueous leaf extracts of Albizia lebbeck induce histological changes of reproductive organs of alloxan induced diabetic albino rats. , SCIREA Journal of Clinical Medicine. Volume 1, Issue 2, December 2016 | PP. 336-353.

References

[ 1 ] Cornell University, college of Agricultural and life sciences (2003) “Saponins” (Online): www.ansci.cornell.edu/plants/toxicagents/saponin.html.
[ 2 ] Mohammad F., Singh P.P., Irchhaiya R.(2012). “Review on albizia lebbeck a potent herbal drug”. International Research Journal of Pharmacy; Vol 3(5): 63-68.
[ 3 ] Shirode DS, Jain BB, Mahendra kumar CB and Setty SR: Hepatoprotective and antioxidant effects of Albizzia lebbeck against thioacetamide induced hepatotoxicity in rats. Journal of Chemical and Pharmaceutical sciences 2012; 5: 199-204.
[ 4 ] Peter J Havel, Tina M. Hahn, Dana K Sindeler, Dennis G. Baskin, Mary F Dallman, David S Weigle and Micheal W Schwartz (2000)“ Effects of streptozotocin induced diabetes insulin treatment on hypothalamic melancortin system and muscle uncoupling protein 3” Diabetes journal. Vol: 49:244-249.
[ 5 ] Kasture VS, Chopde CT, Deshmukh VK. Anticonvulsive activity of Albizzia lebbeck, Hibiscus rosa sinesis and Butea monosperma in experimental animals. J Ethnopharmacol 2000;71: 65-75
[ 6 ] Rakesh C, R. S. Gupta , J. B. S. Kachhaw, D Singh, S. K. Verma. Inhibition of spermatogenesis by Triterpenes of Albizia lebbeck (L.) Benth pods in male albino rats. Journal of natural remedies. Vol. 7/1 (2007) 86 – 93
[ 7 ] Gupta RS, Kachhawa JB, Chaudhary R. Antispermatogenic, antiandrogenic activities of Albizia lebbeck (L.) Benth bark extract in male albino rats. Phytomedicine. 2006 Mar;13 (4):277-83. Epub 2005 Sep 19.
[ 8 ] Carvalho EN, Carvalho NAS, Ferreiri IM (2003). Experimental model of induction of diabetes mellitus in rats. Actacir Bras. 18; 60-64
[ 9 ] Kang TH, Jeong SJ, Kim NY, Higuchi R, Kim YC. Sedative activity of two flavonol glycosides isolated from the flowers of Albizzia julibrissin Durazz. J Ethnopharmacol 2000; 71: 321-3.
[ 10 ] Assis TS, Almeida RN, Barbosa-Filho JM, Medeiros IA. CNS pharmacological effects of the total alkaloidal fraction from Albizzia inopinata leaves. Fitoterapia 2001; 72: 124-30.
[ 11 ] Seetharam YN, Sujeeth H, Jyothishwaran G, Barad A, Sharanabasappa G, Umareddy B, et al. Antifertility effect of ethanolic extract of Amalakyadi churna in male albino mice. Asian J Androl 2003; 5: 247-50.
[ 12 ] Hess, R.A. and L.R. de Franca, 2008. Spermatogenesis and cycle of the seminiferous epithelium. Adv. Exp. Med. Biol., 636: 1-15.
[ 13 ] Osinowo, O.A., 2006. Introduction to Animal Reproduction. 1st Edn., Sophie Academic Services Limited, Abeokuta, Nigeria, pp: 91.
[ 14 ] Gupta, R.S., J.B. Kachhawa and R. Chaudhary, 2004. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats. Asian J. Androl., 6: 155-159.
[ 15 ] Gupta, R.S., R. Chaudhary, R.K. Yadav, S.K. Verma and M.P. Dobhal, 2005. Effect of saponins of Albizia lebbeck (L.) Benth bark on the reproductive system of male albino rats. J. Ethnopharmacol., 96: 31-36.
[ 16 ] Saalu LC, 2016. Nigerian Folklore Medicinal Plants with Potential Antifertility Activity in Males: A Scientific Appraisal. Research Journal of Medicinal Plants, 10: 201-227.