Metabolites of Lycium barbarum L. from Lactobacillus acidophilus as Anti-hepatocellular carcinoma agents: induce apoptosis

Volume 5, Issue 2, April 2020     |     PP. 30-46      |     PDF (957 K)    |     Pub. Date: May 10, 2020
DOI:    206 Downloads     5886 Views  

Author(s)

Jie Liu, The Third Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen 518020, China; School of Medicine, Shenzhen University, Shenzhen 518060, China.
Huailing Wang, The Third Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen 518020, China.
Zhendan He, Department of Pharmacy, Health Science Center, Shenzhen University, Shenzhen 518060, China.
Xiaowei Zeng, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Lin Mei, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Anjin Tao, Hybio Pharmaceutical Co., Ltd. , Shenzhen 518057, China.
Zhigang Liu, The Third Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen 518020, China; School of Medicine, Shenzhen University, Shenzhen 518060, China.
Xizhuo Sun, The Third Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen 518020, China.
Xiaoyu Liu, School of Medicine, Shenzhen University, Shenzhen 518060, China.

Abstract
The fruit of Lycium barbarum L., also known as Gouqi, is a well-known Chinese herbal medicine with various biological activities. Gouqi has a long history of consumption in fermented milk products. In current study, five novel Gouqi metabolites by Lactobacillus acidophilus (GMLs) were structural identified and anti-neoplastic potency against hepatocellular carcinoma (HCC) was further explored. Mechanistic study revealed that GML-4 (methyl 2-(benzyloxy)-2-(2-(2,4-dimethoxybenzamido)acetamido)acetate) blocked the HepG2 cells in G0/G1-phase, as indicated by the decreased expressions of Cyclins and CDKs, and increased expressions of p21 and p27. Further, GML-4-induced cell apoptosis, as indicated by Caspases activation and phosphorylation of AKT/mTOR/S6K1/4E-BP1 signaling pathways. Of note, these findings suggest that GML-4 might be a potential lead compound candidate for the management of anti-HCC.

Keywords
Lactobacillus acidophilus; Lycium barbarum L.; Liver cancer; AKT/mTOR/S6K1/4E-BP1

Cite this paper
Jie Liu, Huailing Wang, Zhendan He, Xiaowei Zeng, Lin Mei, Anjin Tao, Zhigang Liu, Xizhuo Sun, Xiaoyu Liu, Metabolites of Lycium barbarum L. from Lactobacillus acidophilus as Anti-hepatocellular carcinoma agents: induce apoptosis , SCIREA Journal of Chemistry. Volume 5, Issue 2, April 2020 | PP. 30-46.

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