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M. A. El-Badry, Botany and Microbiology Department, Faculty of Science, Al-Azher University
Mohamed Ali El-Badry Hafez Amin, Botany and Microbiology Department, Faculty of Science, Al-Azhar University
The rhizosphere microbes play an important role in improving medicinal values of medicinal plants. The role of microbes in plant growth, nutrient availability, disease resistance, yield and quality of medicinal compounds is demonstrated in medicinal plants. There are increasing interests in the research of the interaction between medicinal plant and their rhizosphere microbes for the improvement of medicinal plants. Dry Rhizosphere of medicinal plants collected form Sinai, Egypt and water treated represents a common physiological stress for the microbial communities residing in surface of these medicinal plants. A dry and wetting by water induce lysis in a significant proportion of the microbial biomass and, for a number of reasons, Effect directly or indirectly on microbial community composition. In this study Dry sand and water treated as common stress in the laboratory by exposing three different rhizosphere immersed in water to 10 day and 20 day period.
The three rhizospheric medicinal plants were collected from Saint Katherine Mountain, Sinai, Egypt. Bacterial community of dry and immersed with distilled water were evaluated every 10 day of incubation. Total DNA was extracted from sand samples and characterized its bacterial communities using the terminal restriction fragment length polymorphism method and degrading gradient Gel electrophoresis. This work revealed that water changed bacterial community in all samples compared with dry sand due to water may induced shifts in bacterial community. Bacterial community clone library of Capparis spinosa rhizosphere were studied and phylogenetic tree of bacterial community were evaluated.
Microbial fingerprint, bacterial community, TRFLP, medicinal plants, soil DNA extraction, 16S ribosomal RNA
Cite this paper
M. A. El-Badry, Mohamed Ali El-Badry Hafez Amin, Bacterial community metagenomic and variation of some medicinal plant rhizosphere collected form Sinai, SCIREA Journal of Agriculture. Vol. 1 , No. 1 , 2016 , pp. 16 - 34 .
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