Antioxidants and bioactivities of total flavonoids Extracted from Kunlun Chrysanthemum Flowers

Volume 3, Issue 3, June 2018     |     PP. 25-49      |     PDF (1272 K)    |     Pub. Date: September 25, 2018
DOI:    1555 Downloads     7797 Views  

Author(s)

Hongyue Zhai, College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China
Siqun Jing, College of food science and Engineering, Shaoguan University, Daxue Road 288, Shaoguan, Guangdong 512005, China
Leping Su, College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China
Zhiyan Ren, College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China
Yinna Wang, College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China
Liang-Jun Yan, Department of Pharmaceutical Sciences, UNT System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX 76107, United States

Abstract
Plant extracts have medicinal and pharmacological values. The aim of the present study was to evaluate the antioxidant activity, antibacterial property, and protective effect on liver injury induced by carbon tetrachloride (CCl4) in mice as well as the antiproliferative effect on tumor cells of Kunlun Chrysanthemum total flavonoids (KCTF). We measured free radical scavenging activities of KCTF and also evaluated KCTF's antioxidative effects on lipid peroxidation and superoxide dismutase activity. Moreover, KCTF's detoxification effect was assessed on acute liver injury in mouse induced by carbon tetrachloride (CCl4) and its anti-proliferation property was determined using HeLa cells and esophageal cancer cells. Results indicated that KCTF had significant antioxidant activity. KCTF inhibited the growth of both Gram positive and Gram negative bacteria. However, KCTF had no significant protective effect on acute liver injury induced by CCl4. Results of the antiproliferative effect of KCTF on HeLa cells and Eca109 cells indicated that KCTF possessed great inhibitory activity with average IC50 values of 133.6±0.1885 μg/mL, 192.0±0.07719 μg/mL, respectively. Therefore, the present study indicates that KCTF may have potential application on functional food industry due to its natural antioxidant, bacteriostatic and antiproliferative effects.

Keywords
Kunlun Chrysanthemum total flavonoids (KCTF); antioxidant activity; antibacterial property; antiproliferative effect; liver injury

Cite this paper
Hongyue Zhai, Siqun Jing, Leping Su, Zhiyan Ren, Yinna Wang, Liang-Jun Yan, Antioxidants and bioactivities of total flavonoids Extracted from Kunlun Chrysanthemum Flowers , SCIREA Journal of Chemistry. Volume 3, Issue 3, June 2018 | PP. 25-49.

References

[ 1 ] Abe, R., Ueo, H., & Akiyoshi, T. (1994). Evaluation of MTT Assay in Agarose for Chemosensitivity Testing of Human Cancers: Comparison with MTT Assay. Oncology, 51(5), 416-425.
[ 2 ] Aron, P. M., & Kennedy, J. A. (2008). Flavan-3-ols: nature, occurrence and biological activity. Molecular Nutrition & Food Research, 52(1), 79.
[ 3 ] Bratkov, Viktor M., Shkondrov, Aleksandar M., Zdraveva, Petranka K., & Krasteva, Ilina N. (2016). Flavonoids from the Genus Astragalus: Phytochemistry and Biological Activity. Pharmacognosy Reviews, 10(19), 11-32.
[ 4 ] Cheng, Y., Qiu, F., Huang, J., Tashiro, S, Onodera, S, & Ikejima, T. (2008). Apoptosis-suppressing and autophagy-promoting effects of calpain on oridonin-induced L929 cell death. Archives of Biochemistry & Biophysics, 475(2), 148-155.
[ 5 ] Dias, T, Bronze, M. R., Houghton, P. J., Motafilipe, H, & Paulo, A. (2010). The flavonoid-rich fraction of Coreopsis tinctoria promotes glucose tolerance regain through pancreatic function recovery in streptozotocin-induced glucose-intolerant rats. Journal of Ethnopharmacology, 132(2), 483-490.
[ 6 ] Fang, Mei Zhu, Chao, Qun Fang, Lan, Yan, Liu, Xiao Ning, Xin, X. U., Fan, Yong Hong, & Zhang, Fu Chun. (2010). Optimization of Flavonoids Extraction from Artemisia rupestris L. by Response Surface Methodology. Food Science, 31(16), 83-86.
[ 7 ] Fang, Ruiping, Tang, Hui, Huang, Jian, Chen, Ruirui, & Zhang, Baoguang. (2009). Research Status of Pharmacological Effects and Analysis Strategies of Nutrients of Snow Chrysanthemum. Journal of Asian Natural Products Research, 11(6), 576-583.
[ 8 ] Galijatovic, Alema, Walle, U. Kristina, & Walle, Thomas. (2000). Induction of UDP-Glucuronosyl-Transferase by the Flavonoids Chrysin and Quercetin in Caco-2 Cells. Pharmaceutical Research, 17(1), 21-26.
[ 9 ] Gao, Fei, Chen, Fang, Qi-Lian, M. A., Hao, Li Li, Liu, Jiang Yun, Ya-Li, L. I., & Yang, Shi Lin. (2016). Simultaneous Determination of Four Flavonoids Components in Coreopsis tinctoria by HPLC. Chinese Journal of Experimental Traditional Medical Formulae.
[ 10 ] Goldar, S, Khaniani, M. S., Derakhshan, S. M., & Baradaran, B. (2015). Molecular mechanisms of apoptosis and roles in cancer development and treatment. Asian Pacific Journal of Cancer Prevention Apjcp, 16(6), 2129-2144.
[ 11 ] Huang, S. S., & Zheng, R. L. (2006). Biphasic regulation of angiogenesis by reactive oxygen species. Pharmazie Die, 61(3), 223-229.
[ 12 ] Ji, Yu Bin, Gao, Shi Yong, Ji, Hong Rui, Kong, Qi, Zhang, Xiu Juan, & Yang, Bao Feng. (2004). Anti-neoplastic efficacy of Haimiding on gastric carcinoma and its mechanisms. 世界胃肠病学杂志(英文版), 10(4), 484-490.
[ 13 ] Jing, S., Ouyang, W., Ren, Z., Xiang, H., & Ma, Z. (2013). The in vitro and in vivo antioxidant properties of Cyperus esculentus oil from Xinjiang, China. Journal of the Science of Food & Agriculture, 93(6), 1505–1509.
[ 14 ] Jing, Si Qun, & Zhang, Xiao Ming. (2013). Optimization of the technology condition of ultra-high pressure on extraction polysaccharides from Coreopsis basalis. Food Science & Technology.
[ 15 ] Jing, Siqun, Chai, Wenjie, Guo, Gai, Zhang, Xiaoming, Dai, Jun, & Yan, Liang-Jun. (2016). Comparison of antioxidant and antiproliferative activity between Kunlun Chrysanthemum flowers polysaccharides (KCCP) and fraction PII separated by column chromatography. Journal of Chromatography B, 1019, 169-177. doi: 10.1016/j.jchromb.2016.01.004
[ 16 ] Jing, Siqun, wang, Saisai, Li, Qian, Zheng, Lian, Yue, Li, Fan, Shaoli, & Tao, Guanjun. (2016). Dynamic high pressure microfluidization-assisted extraction and bioactivities of Cyperus esculentus (C. esculentus L.) leaves flavonoids. Food Chemistry, 192, 319-327. doi: 10.1016/j.foodchem.2015.06.097
[ 17 ] Jing, Siqun, Zhang, Xiaoming, & Yan, Liang-Jun. (2015). Antioxidant Activity, Antitumor Effect, and Antiaging Property of Proanthocyanidins Extracted fromKunlun ChrysanthemumFlowers. Oxidative Medicine and Cellular Longevity, 2015, 1-10. doi: 10.1155/2015/983484
[ 18 ] Karabin, M, Hudcova, T, Jelinek, L, & Dostalek, P. (2015). Biotransformations and biological activities of hop flavonoids. Biotechnology Advances, 33(6), 1063-1090.
[ 19 ] Lai, L., Lai, C. L., Zhao, J. B., & Chen, J. H. (2010). [Preparation of Ginkgo biloba extract 50-phospholipid complex and study on its physicochemical properties]. Journal of Chinese Medicinal Materials, 33(10), 1624.
[ 20 ] Li, Yali, Chen, Xinmei, Xue, Jie, Liu, Jiangyun, Chen, Xinhua, & Wulasihan, Muhuyati. (2014). Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein. Lipids in Health & Disease, 13(1), 193.
[ 21 ] Liang, Shu Hong, HAMulati, Pang, Shi Bin, & Sun, Yu Hua. (2010). Experimental Study on Hypertensive Effects of Extracts of Coreopsis basalis. Lishizhen Medicine & Materia Medica Research.
[ 22 ] Mancang, Zhao, Wenqing, Jing, Zhang, Juan, . . . Ping. (2009). Impact of MTT based tumor chemosensitivity assay in vitro. The Chinese-German Journal of Clinical Oncology, 8(9), 546-548.
[ 23 ] Meurer-Grimes, Barbara. (1995). New evidence for the systematic significance of acylated spermidines and flavonoids in pollen of Higher Hamamelidae. Brittonia, 47(2), 130-142.
[ 24 ] Pintus, Gianfranco, Cai, Hao, Xie, Zhiyong, Liu, Guanghui, Sun, Xiuman, Peng, Guangtian, . . . Liao, Qiongfeng. (2014). Isolation, Identification and Activities of Natural Antioxidants from Callicarpa kwangtungensis Chun. PLoS ONE, 9(3), e93000. doi: 10.1371/journal.pone.0093000
[ 25 ] Sang, Joon Lee, Kang, Mi Sun, Oh, Jong Suk, Na, Hee Sam, You, Jin Lim, Jeong, Young Il, & Lee, Hyun Chul. (2013). Caffeic acid-conjugated chitosan derivatives and their anti-tumor activity. Archives of Pharmacal Research, 36(12), 1437-1446.
[ 26 ] Sheng-Bin, W. U., & Xie, Jing. (2013). Research progress of natural food preservatives from plant materials. Guangdong Agricultural Sciences, 40(23), 110-113.
[ 27 ] Sun, Li, Luo, Qiang, & Zhang, Li. (2011). Effects of Trollius flavonoids on growth and apoptosis of A549 cells. Chinese Journal of Gerontology, 31(1), 82-83.
[ 28 ] Swenson, J. M., Wong, B, Simor, A. E., Thomson, R. B., Ferraro, M. J., Hardy, D. J., . . . Traczewski, M. (2010). Multicenter study to determine disk diffusion and broth microdilution criteria for prediction of high- and low-level mupirocin resistance in Staphylococcus aureus. Journal of Clinical Microbiology, 48(7), 2469-2475.
[ 29 ] Wang, H. B. (2014). Cellulase-assisted extraction and antibacterial activity of polysaccharides from the dandelion Taraxacum officinale. Carbohydrate Polymers, 103(1), 140-142.
[ 30 ] Xiao, J., Sun, J., Yao, L., Zhao, Q., Wang, L., Wang, X., . . . Zhao, B. (2012). Physicochemical characteristics of ultrasonic extracted polysaccharides from cordyceps cephalosporium mycelia. International Journal of Biological Macromolecules, 51(1–2), 64-69.
[ 31 ] Yan, X. U., & Liu, De Qing. (2007). Study on the method of extracting natural preservative in pepper and its bacteriostasis. China Condiment.
[ 32 ] Yang, Xiaolin, Wang, Rufeng, Zhang, Shaopeng, Zhu, Wenjun, Tang, Jin, Liu, Jianfang, . . . Zheng, Yonglian. (2014). Polysaccharides from Panax japonicus C.A. Meyer and their antioxidant activities. Carbohydrate Polymers, 101, 386-391. doi: 10.1016/j.carbpol.2013.09.038
[ 33 ] Yang, Yingshi, Chen, Wei, Yang, Haiyan, & Xin, Zhihong. (2014). Isolation and identification of two flavonoids compounds from Coreopsis tinctoria Nutt.and evaluation of their antioxidant activities. Journal of Nanjing Agricultural University.
[ 34 ] Yao, Qing, Wu, Chuan Fang, Luo, Ping, Xiang, Xiao Cong, Liu, Jun Jie, Mou, Lin, & Bao, Jin Ku. (2010). A new chitin-binding lectin from rhizome of Setcreasea purpurea with antifungal, antiviral and apoptosis-inducing activities. Process Biochemistry, 45(9), 1477-1485.
[ 35 ] ZHANG, Aytulun·Simayil. (2010). Study on content determination of Kunlun Chrysanthemum polysaccharides from Uygur medicine by phenol-sulfuric acid method. Chinese Journal of Pharmaceutical Analysis.
[ 36 ] Zhang, Chun Ling, Li-Jun, W. U., Shin-Ichi, Tashiro, Satoshi, Onodera, & Takashi, Ikejima. (2003). Oridonin induced HeLa cell apoptosis through capase pathway. Chinese Pharmacological Bulletin, 19(5), 521-525.
[ 37 ] Zhang, X. Y., Li, W. G., Wu, Y. J., Bai, D. C., & Liu, N. F. (2005). Proanthocyanidin from grape seeds enhances doxorubicin-induced antitumor effect and reverses drug resistance in doxorubicin-resistant K562/DOX cells. Canadian Journal of Physiology & Pharmacology, 83(3), 309.
[ 38 ] Zhao, Yan Hong, Jian-Ke, L. I., Zhao, Wei, & Xiao-Xia, W. U. (2009). Evaluation on Antioxidant Activities of Extracts from Common Edible and Medicinal Plants in vitro. Food Science, 30(3), 104-108.
[ 39 ] Zhong, Mingli, Gao, Ya, Chen, Yifei, & Zhang, Kefeng. (2015). Protective Effects of Total Flavonoids in Polygonum perfoliatum L.on the Acute Liver Injury of Mice Induced by CCl_4 and Its Action Mechanism. Medicinal Plant(Z2).
[ 40 ] Zhou, Jian Xin. (2006). Reviews on Research Progresses, Actual Problem and Prospects on Natural Food Preservatives from Plant Materials. Food Science, 27(1), 263-268.