IN VIVO ELUCIDATION OF ANTI-ARTHRITIC EFFECT OF PLANT ALKALOID BERERINE IN EXPERIMENTAL MOUSE JOINT INFLAMMATION

Volume 4, Issue 1, February 2019     |     PP. 1-15      |     PDF (1739 K)    |     Pub. Date: March 23, 2019
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Author(s)

Lyudmila Belenska-Todorova, Medical Faculty, Sofia University, Bulgaria
Petya Ganova, Institute of Microbiology, Department of Immunology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Nina Ivanovska, Institute of Microbiology, Department of Immunology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Abstract
Berberine is a protoberberine alkaloid possessing various pharmacological activities, known as a Janus kinase (JAK) inhibitor. The aim of the present study was to elucidate the mechanism of its action in relation to JAK2/3 and sclerostin expression in synovium and cartilage, the presence of Receptor activator of factor kΒ Ligand (RANKL) positive cells in synovial fluid, the presence of dendritic cells (DC) in cartilage and the expression of TNF-related apoptosis-inducing ligand (TRAIL) and its receptor DR5 on bone marrow (BM) cells. The experiments were conducted in a mouse model of erosive inflammatory joint disease, relevant to human rheumatoid arthritis, induced through intraarticular injection of zymosan. Berberine decreased the phosphorilation of both JAK2 and JAK3 in arthritic joints, the expression of RANKL, TRAIL and DR5. The alkaloid did not affect sclerostin expression and prevented the decrease of the number of 33D1 positive dendritic cells. Present results contribute to more clear understanding of the ameliorative effect of berberine on erosive processes in joint inflammation.

Keywords
berberine, JAK inhibitors, erosive arthritis, dendritic cells

Cite this paper
Lyudmila Belenska-Todorova, Petya Ganova, Nina Ivanovska, IN VIVO ELUCIDATION OF ANTI-ARTHRITIC EFFECT OF PLANT ALKALOID BERERINE IN EXPERIMENTAL MOUSE JOINT INFLAMMATION , SCIREA Journal of Biology. Volume 4, Issue 1, February 2019 | PP. 1-15.

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