Pathogenicity of various Mycoplasma gallisepticum strains in vaccinated and non-vaccinated breeders flocks’ chicken embryos

Author(s)

Abstract
This study was undertaken to determine the pathogenicity of six of Mycoplasma gallisepticum (MG) local field strains in specific pathogen free and commercial broiler breeder chicken embryos and the appropriate viscera organs for sampling in hatched chickens for detection and isolation of Mycoplasma gallisepticum. Three hundred eighty (380) each embryonated eggs were grouped into three, according to reference strains (MGS6 and ts-11), field isolates and control (uninoculated and broth inoculated) groups. The embryonated eggs were each inoculated with 0.2ml “pleuropneumonia like organism” (PPLO) broth containing 6.2 x 10 CCU/ml of reference strain or field isolate respectively, via yolk sac, at day 6 of incubation and were examined at necropsy days 7, 10, 13 and 15 postinoculation. Grossly, reference and field MG infection groups showed more significant lesion findings when compared to control groups in SPF embryonated eggs however not significant in commercial broiler embryonated eggs. Postmortem findings of inoculated embryos for both type embryos were: dwarfing, curled toes, head edema, slightly enlarged and pale liver, slightly enlarged and pale spleen and green colour liver. Histopathological lesions of embryos from MG inoculated groups of all sources revealed that there were mild to severe infiltration of inflammatory cells such as neutrophils and lymphocytes. Caseous air sac lesion score ranged from 0 to 1 for SPF embryos and 1 to 3 lesion score for commercial broiler embryos which might be due to the formation of antigen-antibodies complexes.

Keywords
Mycoplasma gallisepticum, pathogenicity, Caseous airsac, Embryonated eggs

Cite this paper
Ching Giap Tan, Choon Howe Hoo, Aini Ideris, Mohd Hair-Bejo, Abdul Rahman Omar, Stanley H. Kleven, Pathogenicity of various Mycoplasma gallisepticum strains in vaccinated and non-vaccinated breeders flocks’ chicken embryos , SCIREA Journal of Animal Husbandry and Veterinary Medicine. Volume 1, Issue 1, October 2016 | PP. 1-22.

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