Human Serum Albumin And Hydroxy Ethyl Starch Are Protective Alone And In Combination For Human Hematopoietic Stem Cells Stored at -80ºC With 5% Dimethylsulphoxide

Volume 7, Issue 1, February 2023     |     PP. 1-10      |     PDF (970 K)    |     Pub. Date: January 1, 2023
DOI: 10.54647/pm31186    97 Downloads     105989 Views  

Author(s)

Ivan Tonev, National Specialized Hospital for Active Treatment of Hematological Diseases, Sofia, Bulgaria
Milcho Mincheff, National Specialized Hospital for Active Treatment of Hematological Diseases, Sofia, Bulgaria

Abstract
The preservation of hematopoietic stem cells (HSC), which allows postponing of their transplantation is by cryoprotection. Dimethyl sulfoxide (DMSO) is a widely used intracellular cryoprotectant which is toxic for cells and patients at temperature above 0 ºC. A possible approach to reduce these toxicities is with addition of extracellular cryoprotectors as hydroxyethyl starch (HES) and plasma proteins to allow the use of DMSO in lower final concentration. We tested the protective role of HES and the plasma protein human serum albumin (HSA), a for human hematopoietic stem cells, subjected either to osmotic stress (a major factor in cellular injury during slow freezing) or to cryopreservation by unprogrammed freezing and storage at -80ºC. The viability was tested by trypan-blue exclusion assay.
HES has a protective effect on HSCs both against osmotic stress and cryopreservation and reduces cell aggregation. Addition of HSA to the cryoprotective solution improves viability and reduces cell clumping after thawing. Cryopreservation of HSCs with final 5% DMSO concentrations can be optimized by the addition of extracellular agents such as HES and HSA. This reduces DMSO toxicity to both HSCs and to patients during transplantation.

Keywords
DMSO concentration, extracellular cryoprotectors, -80 degrees, hydroxyethylstarch, HES, human albumin, HAS, hematopoietic stem cells, HSCs

Cite this paper
Ivan Tonev, Milcho Mincheff, Human Serum Albumin And Hydroxy Ethyl Starch Are Protective Alone And In Combination For Human Hematopoietic Stem Cells Stored at -80ºC With 5% Dimethylsulphoxide , SCIREA Journal of Medicine. Volume 7, Issue 1, February 2023 | PP. 1-10. 10.54647/pm31186

References

[ 1 ] Rowley, S.D., Hematopoietic Stem Cell Cryopreservation: A Review of Current Techniques. Journal of Hematotherapy, 1992. 1(3): p. 233-250.
[ 2 ] Sawai, M., et al., Reversible G1 arrest in the cell cycle of human lymphoid cell lines by dimethyl sulfoxide. Exp Cell Res, 1990. 187(1): p. 4-10.
[ 3 ] Windrum, P., et al., Variation in dimethyl sulfoxide use in stem cell transplantation: a survey of EBMT centres. Bone Marrow Transplant, 2005. 36(7): p. 601-3.
[ 4 ] Zambelli, A., et al., Clinical toxicity of cryopreserved circulating progenitor cells infusion. Anticancer Res, 1998. 18(6b): p. 4705-8.
[ 5 ] Davis, J.M., et al., Clinical toxicity of cryopreserved bone marrow graft infusion. Blood, 1990. 75(3): p. 781-6.
[ 6 ] Zenhausern, R., et al., Fatal cardiac arrhythmia after infusion of dimethyl sulfoxide-cryopreserved hematopoietic stem cells in a patient with severe primary cardiac amyloidosis and end-stage renal failure. Ann Hematol, 2000. 79(9): p. 523-6.
[ 7 ] Takeda, K., et al., Respiratory Toxicity of Dimethyl Sulfoxide, in Respirology, M. Pokorski, Editor. 2016, Springer International Publishing: Cham. p. 89-96.
[ 8 ] Higman, M.A., et al., Reversible leukoencephalopathy associated with re-infusion of DMSO preserved stem cells. Bone Marrow Transplant, 2000. 26(7): p. 797-800.
[ 9 ] Hornberger, K., et al., Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes. Transfusion Medicine and Hemotherapy, 2019. 46(3): p. 188-196.
[ 10 ] Stiff, P.J., et al., Autologous bone marrow transplantation using unfractionated cells cryopreserved in dimethylsulfoxide and hydroxyethyl starch without controlled-rate freezing. Blood, 1987. 70(4): p. 974-8.
[ 11 ] Leibo, S.P., et al., Effects of preezing on marrow stem cell suspensions: Interactions of cooling and warming rates in the presence of pvp, sucrose, or glycerol. Cryobiology, 1970. 6(4): p. 315-332.
[ 12 ] Meryman, H.T., Cryoprotective agents. Cryobiology, 1971. 8(2): p. 173-183.
[ 13 ] Fernandez, O., et al., Trehalose and plant stress responses: friend or foe? Trends Plant Sci, 2010. 15(7): p. 409-17.
[ 14 ] Storey, K.B., Life in a frozen state: adaptive strategies for natural freeze tolerance in amphibians and reptiles. Am J Physiol, 1990. 258(3 Pt 2): p. R559-68.
[ 15 ] Crowe, J.H., L.M. Crowe, and D. Chapman, Preservation of membranes in anhydrobiotic organisms: the role of trehalose. Science, 1984. 223(4637): p. 701-3.
[ 16 ] Stolzing, A., et al., Hydroxyethylstarch in cryopreservation – Mechanisms, benefits and problems. Transfusion and Apheresis Science, 2012. 46(2): p. 137-147.
[ 17 ] Crowe, J.H., et al., Interactions of sugars with membranes. Biochim Biophys Acta, 1988. 947(2): p. 367-84.
[ 18 ] Yu, G. and R. Li, Interfacial Interactions of Sucrose during Cryopreservation Detected by Raman Spectroscopy. 2019. 35(23): p. 7388-7395.
[ 19 ] Takahashi, T., et al., Mechanism of cryoprotection by extracellular polymeric solutes. Biophys J, 1988. 54(3): p. 509-18.
[ 20 ] Meryman, H.T., Cryopreservation of living cells: principles and practice. Transfusion, 2007. 47(5): p. 935-45.
[ 21 ] Tonev, I. and M. Mincheff, Comparison of viability and hematologic recovery after allogeneic transplantations with fresh or frozen in 5% dimethylsulphoxide (dmso) hematopoietic stem cells (hsc). HemaSphere, 2021: p. 843-843.
[ 22 ] Hayakawa, J., et al., 5% dimethyl sulfoxide (DMSO) and pentastarch improves cryopreservation of cord blood cells over 10% DMSO. Transfusion, 2010. 50(10): p. 2158-66.
[ 23 ] Westphal, M., et al., Hydroxyethyl starches: different products--different effects. Anesthesiology, 2009. 111(1): p. 187-202.
[ 24 ] Treib, J., et al., Increased haemorrhagic risk after repeated infusion of highly substituted medium molecular weight hydroxyethyl starch. Arzneimittelforschung, 1997. 47(1): p. 18-22.
[ 25 ] Bernard, C., et al., Hydroxyethylstarch and osmotic nephrosis-like lesions in kidney transplants. The Lancet, 1996. 348(9041): p. 1595.
[ 26 ] Ragab, A.H., E. Gilkerson, and M. Myers, Factors in the cryopreservation of bone marrow cells from children with acute lymphocytic leukemia. Cryobiology, 1977. 14(2): p. 125-134.
[ 27 ] Knight, S.C., J. Farrant, and L.E. McGann, Storage of human lymphocytes by freezing in serum alone. Cryobiology, 1977. 14(1): p. 112-115.
[ 28 ] Clarke, D.M., et al., Improved post-thaw recovery of peripheral blood stem/progenitor cells using a novel intracellular-like cryopreservation solution. Cytotherapy, 2009. 11(4): p. 472-479.
[ 29 ] Gonçalves, A.D., et al., The effect of protein concentration on the viscosity of a recombinant albumin solution formulation. RSC Advances, 2016. 6(18): p. 15143-15154.
[ 30 ] Lionetti, F.J., S.M. Hunt, and P.S. Lin, Improved method for the cryopreservation of human red cells in liquid nitrogen with hydroxyethyl starch. Cryobiology, 1976. 13(5): p. 489-99.
[ 31 ] Malinin, T.I.P., Vernon P., Toxicity of dimethyl sulfoxide on HeLa cells. Cryobiology, 1967. 4(2): p. 90-96.