Volume 1, Number 2 (2016)
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Home > Journals > SCIREA Journal of Biology > Archive > Paper Information

Methods and techniques to evaluate sciatic nerve recovery in rats

Volume 1, Issue 2, December 2016    |    PP. 66-78    |PDF (167 K)|    Pub. Date: December 23, 2016
220 Downloads     1279 Views  

Author(s)
Kollarou Anna, Democritus University of Thrace, Department of Molecular Biology and Genetics, Alexandroupoli, Greece

Abstract
Quantitative approaches for the evaluation of functional outcome are required in order to develop strategies to improve the regeneration of peripheral nerves. Most of these studies use rats as animal experimental model, as they are inexpensive and easy to work with. In such studies, regeneration can be assessed by numerous methods, including histomorphometry, electrophysiology and gait analysis. Because the sciatic nerve represents the most used one in experimental approaches, testing procedures for the assessment of its function after injuries have been developed. To evaluate the degree of functional loss, a footprint analysis method has been introduced and modified over subsequent years. The method uses indices of hind limb performance showing high degree of correlation with functional recovery. Data support a characterization of the sciatic nerve crush injury that will allow the study of peripheral nerve regeneration in the presence of neuroprotective agents in posttraumatic nerve repair.

Keywords
rat sciatic nerve, functional nerve recovery, walking track analysis, gait analysis, nerve morphometry

Cite this paper
Kollarou Anna, Methods and techniques to evaluate sciatic nerve recovery in rats, SCIREA Journal of Biology. Vol. 1 , No. 2 , 2016 , pp. 66 - 78 .

References

[ 1 ] Bauer T, Resnick L. Peculiar problems affecting the sciatic nerve. JBJS Case Connect. 2016; 6(4): e84.
[ 2 ] Detloff MR, Fisher LC, Deibert RJ, Basso DM. Acute and chronic tactile sensory testing after spinal cord injury in rats. J Vis Exp. 2012; 62: e3247.
[ 3 ] Ganga MVM, Coutinho-Netto J, Colli BO, Marques Junior W, Catalão CHR, Santana RT, Oltramari MRP, Carraro KT, Lachat J-J, da Silva Lopes L. Sciatic nerve regeneration in rats by a nerve conduit engineering with a membrane derived from natural latex. Acta Bras Cir. 2012; 27(12): 885–891.
[ 4 ] Mackinnon SE, Hudson AR, Hunter DA. Histologic assessment of nerve regeneration in the rat. Plast Reconstr Surg. 1985; 75(3): 384–388.
[ 5 ] Dellon AL, Mackinnon SE. Selection of the appropriate parameter to measure neural regeneration. Ann Plast Surg. 1989; 23(3): 197–202.
[ 6 ] Pereira T, Gärtner A, Amorim I, Almeida A, Caseiro AR, Armada-da-Silva PAS, Amado S, Fregnan F, Varejão ASP, Santos JD, Bartolo PJ, Geuna S, Luís AL, Mauricio AC. Promoting nerve regeneration in a neurotmesis rat model using poly(dl-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton’s jelly: In vitro and in vivo analysis. BioMed Res Int. 2014; 17 p.
[ 7 ] Varejão AS, Melo-Pinto P, Meek MF, Filipe VM, Bulas-Cruz J. Methods for the experimental functional assessment of rat sciatic nerve regeneration. Neurol Res. 2004; 26(2): 186–194.
[ 8 ] Petrova ES. Injured nerve regeneration using cell-based therapies: Current challenges. Acta Naturae. 2015; 7(3): 38–47.
[ 9 ] Dellon AL, Mackinnon SE. Selection of the appropriate parameter to measure neural regeneration. Ann Plast Surg. 1989; 23(3): 197–202.
[ 10 ] Evans PJ, Bain JR, Mackinnon SE, Makino AP, Hunter DA. Selective reinnervation: a comparison of recovery following microsuture and conduit nerve repair. Brain Res. 1991; 559(2): 315–321.
[ 11 ] Dellon AL, Mackinnon SE. Sciatic nerve regeneration in the rat. Validity of walking track assessment in the presence of chronic contractures. Microsurgery. 1989; 10(3): 220–225.
[ 12 ] Breshah MN, Sadakah AA, Eldrieny EA, Saad KA. Functional and histological evaluation of rat sciatic nerve anastomosis using cyanoacrylate and fibrin glue. Tanta Dental Journal, 2013; 10: 67–74.
[ 13 ] Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, et al. Interleukin-1 beta promotes sensory nerve regeneration after sciatic nerve injury. Neurosci Lett 2008, 440(2): 130–133.
[ 14 ] Guilbaud G, Benoist JM, Levante A, Gautron M, Wilier JC. Primary somatosensory cortex in rats with pain-related behaviours due to a peripheral mononeuropathy after moderate ligation of one sciatic nerve: neuronal responsivity to somatic stimulation. Exp Brain Res. 1992; 92(2): 227–245.
[ 15 ] Munro CA, Szalai JP, Mackinnon SE, Midha R. Lack of association between outcome measures of nerve regeneration. Muscle Nerve. 1998; 21(8): 1095–1097.
[ 16 ] Hadlock TA, Koka R, Vacanti JP, Cheney ML. A comparison of assessments of functional recovery in the rat. J Peripher Nerv Syst. 1999; 4(3-4): 258–264.
[ 17 ] Varejão AS, Cabrita AM, Meek MF, Bulas-Cruz J, Gabriel RC, Filipe VM, Melo-Pinto P, Winter DA. Motion of the foot and ankle during the stance phase in rats. Muscle and Nerve. 2002; 26(5): 630–635.
[ 18 ] Sarikcioglu L, Demirel BM, Utuk A. Walking track analysis: an assessment method for functional recovery after sciatic nerve injury in the rat. Folia Morphol. 2009; 68(1): 1–7.
[ 19 ] Mackinnon SE, Dellon AL, O’Brien JP. Changes in nerve fiber numbers distal to a nerve repair in the rat sciatic nerve model. Muscle Nerve. 1991; 14(11): 1116–1122.
[ 20 ] Nichols CM, Myckatyn TM, Rickman SR, Fox IK, Hadlock T, Mackinnon SE. Choosing the correct functional assay: a comprehensive assessment of functional tests in the rat. Behav Brain Res. 2005; 163(2):143–158.
[ 21 ] Gutmann E, Gutmann L. Factors affecting recovery of motor function after nerve lesions. J Neurol Psych. 1942; 5: 117–129.
[ 22 ] Hasegawa K. A new method of measuring functional recovery after crushing the peripheral nerves in unanesthetized and unrestrained rats. Experientia. 1978; 34(2): 272–273.
[ 23 ] Hruska RE, Kennedy S, Silbergeld EK. Quantitative aspects of normal locomotion in rats. Life Sci. 1979; 25(2): 171–180.
[ 24 ] Jolicoeur FB, Rondeau DB, Hamel E, Butterworth RF, Barbeau A. Measurement of ataxia and related neurological signs in the laboratory rat. Can J Neurol Sci. 1979; 6(2): 209-215.
[ 25 ] de Medinaceli L, Freed WJ, Wyatt RJ. An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol. 1982; 77(3): 634–643.
[ 26 ] Carlton JM, Goldberg ΝH. Quantitating integrated muscle function following reinnervation. Plast Reconstr Surg Forum. 1986; 37: 611-612.
[ 27 ] Bain JR, Mackinnon SE, Hunter DA. Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg. 1989; 83(1): 129–138.
[ 28 ] de Medinaceli L, de Renzo E, Wyatt RJ. Rat sciatic functional index data management system with digitized input. Comput Biomed Res. 1984; 17(2): 185–192.
[ 29 ] Bervar M. Video analysis of standing -an alternative footprint analysis to assess functional loss following injury to the rat sciatic nerve. J Neurosci Methods. 2000; 102(2): 109–116.
[ 30 ] Takhtfooladi MA, Jahanbakhsh F, Takhtfooladi HA, Yousefi K, Allahverdi A. Effect of low-level laser therapy (685 nm, 3 J/cm2) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci. 2015; 30(1): 6 p.
[ 31 ] Grasso G, Sfacteria A, Brines M, Tomasello F. A new computed-assisted technique for experimental sciatic nerve function analysis. Med Sci Monit. 2004; 10(1): BR1–BR3.
[ 32 ] Bozkurt A, Tholl S, Wehner S, Tank J, Cortese M, O’Dey DM, Deumens R, Lassner F, Schügner F, Gröger A, Smeets R, Brook G, Pallua N. Evaluation of functional nerve recovery with Visual-SSI Visual-SSI – A novel computerized approach for the assessment of the static sciatic index (SSI). J Neurosci Methods. 2008; 170(1): 117–122.
[ 33 ] Răducan A, Mirică S, Duicu O, Răducan S, Muntean D, Fira-Mlădinescu O, Lighezan R. Morphological and functional aspects of sciatic nerve regeneration after crush injury. Rom J Morphol Embryol. 2013; 54(3 Suppl): 735–739.
[ 34 ] Margiana R, Jusuf AA, Aman RA, Liem IK, Pawitan JA. A new method in walking analysis using the angles around the midpoint between print length and toe spread by four different color footprints. Int J Sci: Basic Appl Res (IJSBAR). 2015; 21(1): 117–128.
[ 35 ] Dijkstra JR, Meek MF, Robinson PH, Gramsbergen A. Methods to evaluate functional nerve recovery in adult rats: Walking track analysis, video analysis and withdrawal reflex. J Neurosci. Methods. 2000; 96(2): 89–96.
[ 36 ] Lowdon IM, Seaber AV, Urbaniak JR. An improved method of recording rat tracks for measurement of the sciatic functional index of de Medinaceli. J Neurosci Methods. 1988; 24(3): 279–281.
[ 37 ] Rushton R, Steinberg H, Tinson C. The effects of a single experience on subsequent reactions to drugs. Br J Pharmacol. 1963; 20(1): 99–105.
[ 38 ] Zellem RT, Miller DW, Kenning JA, Hoenig EM, Buchheit WA. Experimental peripheral nerve repair: environmental control directed at the cellular level. Microsurgery. 1989; 10(4): 290–301.
[ 39 ] Johnston RB, Zachary L, Dellon AL, Seiler WAt, Teplica DM. Improved imaging of rat hindfoot prints for walking track analysis. J Neurosci Methods. 1991; 38(2-3): 111–114.
[ 40 ] Shen N, Zhu J. Application of sciatic functional index in nerve functional assessment. Microsurgery. 1995; 16(8): 552–555.
[ 41 ] Vleggeert-Lankamp CLAM. The role of evaluation methods in the assessment of peripheral nerve regeneration through synthetic conduits: a systematic review. J Neurosurg. 2007; 107: 1168–1189.
[ 42 ] Westerga J, Gramsbergen A. The development of locomotion in the rat. Dev Brain Res. 1990; 57(2): 163–174.
[ 43 ] Walker JL, Evans JM, Meade P, Resig P, Sisken BF. Gait-stance duration as a measure of injury and recovery in the rat sciatic nerve model. J Neurosci Methods. 1994; 52(1): 47–52.
[ 44 ] Inserra MM, Bloch DA, Terris DJ. Functional indices for sciatic, peroneal, and posterior tibial nerve lesions in the mouse. Microsurgery 1998; 18: 119–124.

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