REACTIVE MORPHOLOGICAL CHANGES OF RATS’ HEARTS WITH AN EXPERIMENTAL UNDIFFERENTIATED DYSPLASIA OF CONNECTIVE TISSUE

Volume 5, Issue 5, October 2020     |     PP. 89-107      |     PDF (521 K)    |     Pub. Date: September 22, 2020
DOI:    200 Downloads     3454 Views  

Author(s)

Hryhorieva O. A., Normal Anatomy, Operative Surgery and Topographic Anatomy Department, Zaporizhzhya State Medical University, Zaporizhzhya, Ukraine
Guminskiy Yu.Yo., Normal Anatomy Department, National Pirogov Memorial Medical University, Vinnitsa, Ukraine
Chernyavskiy A. V., Normal Anatomy, Operative Surgery and Topographic Anatomy Department, Zaporizhzhya State Medical University, Zaporizhzhya, Ukraine
Tavrog M.L., Normal Anatomy, Operative Surgery and Topographic Anatomy Department, Zaporizhzhya State Medical University, Zaporizhzhya, Ukraine
Zinich O.L., Normal Anatomy, Operative Surgery and Topographic Anatomy Department, Zaporizhzhya State Medical University, Zaporizhzhya, Ukraine

Abstract
In modern literature, there are sufficient data concerning clinical manifestations of connective tissue dysplasia, its diagnosis, principles of correction, but works devoted to factors that lead to dysplasia, and morphological manifestations of this condition are insufficient.The aim: to establish a reactive morphological changes of the heart of rats with an experimental undifferentiated dysplasia of connective tissue.Materials and methods. As an experimental model of undifferentiated dysplasia of connective tissue it is selected a model of intrafetal antigens injection at the 18 th day of dated pregnancy. The object of the study was 144 hearts of white laboratory rats, which were divided into 3 groups: - The 1st one – intact animals; the 2nd group consisted of experimental animals; the 3rd group consisted of control rats. Histochemical, histological methods, statistic methods were used in the work. It is settled that in rats with an experimental undifferentiated dysplasia of connective tissuethe thinning of the hearts’ walls from birth up to the 45th day of life is defined compared to control, it is most pronounced at the 1st and 9th day of life. In the group of experimental rats, starting from the 21st day of life up to the end of the observation period the relative area occupied by connective tissue fibers is lower compared to control. This happens mainly due to the lower proportion of collagen fibers type III from the 14th up to the 45th day of life. In experimental rats with undifferentiated dysplasia of connective tissue a significant thinning of the intima-media complex of the arteries of the heart was observed throughout the observation period with the most pronounced changes at the 3rd (3,35±0,09 µm vs 4,95±1.03 µm in controls) and the 21st (7,57±0,25 µm vs. 9.5±of 1.62 µm in controls) days of life, p<0.01.

Keywords
undifferentiated dysplasia of connective tissue, heart, collagen fibers, lymphocytes, intrafetal antigen injection

Cite this paper
Hryhorieva O. A., Guminskiy Yu.Yo., Chernyavskiy A. V., Tavrog M.L., Zinich O.L., REACTIVE MORPHOLOGICAL CHANGES OF RATS’ HEARTS WITH AN EXPERIMENTAL UNDIFFERENTIATED DYSPLASIA OF CONNECTIVE TISSUE , SCIREA Journal of Clinical Medicine. Volume 5, Issue 5, October 2020 | PP. 89-107.

References

[ 1 ] Antonyuk V. A. Lectins and their sources of raw materials: monograph. Lviv : Kvart, 2005. 554 p.
[ 2 ] Antunes M, Scirè CA, Talarico R et al. Undifferentiated connective tissue disease: state of the art on clinical practice guidelines / RMD Open, 2018; 4: e000786. https://doi.org/10.1136/rmd open-2018-000786.
[ 3 ] Carpinello OJ., DeCherney AH, Hill MJ. Developmental Origins of Health and Disease: The History of the Barker Hypothesis and Assisted Reproductive Technology. Semin. Reprod. Med. 2018; 36 (3-4): 177–182. DOI : 10.1055/s-0038-1675779.
[ 4 ] Conradt E, Adkins DE, Crowell SE et al. Incorporating epigenetic mechanisms to advance fetal programming theories. Dev. Psychopathol. 2018; 30 (3): 807–824. DOI : 10.1017/S0954579418000469.
[ 5 ] Cooke RF. Effects on Animal Health and Immune Function. Vet. Clin. North Am. Food Anim. Pract. 2019; 35 (2): 331–341. DOI : 10.1016/j.cvfa.2019.02.004.
[ 6 ] D’Alto M, Riccardi A, Argiento P et al. Cardiac involvement in undifferentiated connectІІІe tissue disease at risk for systemic sclerosis (otherwise referred to as very early–early systemic sclerosis): a TDI study. Clin. Exp. Med. 2018; 18 (2): 237–243. DOI : 10.1007/s10238-017-0477-y.
[ 7 ] Danilchenko LI. Scientific substantiation of priority directions of development of medical care of cardiac patients in the city. The World of Medicine and Biology. 2017; 13- 2 (60): 34-39.
[ 8 ] Fesenko MT, Kazakevitch VK, Zyuzin LS, et al. Clinical and instrumental characteristics of small anomalies of development of heart (Mars) in children of Poltava. Contemporary Pediatrics. 2017; 4 (84): 82-85. DOI: 10.15574/SP.2017.84.82. (In Russian)
[ 9 ] Gandzyuk VA, Dyachuk DD, Kondratyuk NYu. Dynamics of morbidity and mortality due to blood circulatory diseases in Ukraine (regional aspect). Bulletin of Problems Biology and Medicine. 2017;2:319–323.
[ 10 ] Hryhorieva OA, Chernyavsky AV. Changes in nuclear-cytoplasmic ratio of cardiomyocytes in heart of rats in the postnatal period after intrauterine antigen introduction. International Trends in Science and Technology : collection of scientific works. article XII Intern. Sciences.-pract. Conf. (M. Warsaw, 30 APR. 2019). Warsaw, 2019; 2: 24-27.
[ 11 ] Hryhorieva OA, Matveyshina TM, Hrinivetska NV. et al. Intrafetal injection of antigens as an experimental model of undifferentiated dysplasia of connective tissue. Medico-biological aspects and multidisciplinary integration in the concept of human health : proceedings of Ukr. Conf. with int. Participation (Ternopil, 9-11 April 2020.) in III parts/ Ternopil. Nat.Med.Univ named after I Horbachevsky MPH of Ukraine. – Ternopil : TNMU, 2020. –Part I. - Pp. 37-40.
[ 12 ] Hryhorieva OA, Voloshyn NA. Experimental model of undifferentiated dysplasy of connective tissue by impaired antigen homeostasis in the system of mother-placenta-foetus. Pathologia 2011; 8(2): 39-42.
[ 13 ] Hryhorieva OA, Skakovsky EG. Morphological characteristics of the formation of the joint capsule of rats in postnatal period in norm and experiment. Bulletin of problems of biology and medicine. 2017. 2. P. 279-286. (In Ukrainian).
[ 14 ] Inherited and multifactor impairments of connective tissue in children. Diagnostic algorythms. Medical Bulletin of the North Caucasus. 2015. Т. 10, № 1. P. 5–35.
[ 15 ] Khait GY, Nechayev EV, GusevS. Structural and functional remodeling of the left ventricle by dysplasia of connective tissue of the heart. Medical Bulletin of the North Caucasus. 2006; 4: 35-40. (In Russian)
[ 16 ] Kuznetsov VA, Soldatov AM, Pankov AV. The Relationship of small anomalies of development of the connective tissue of the heart with the risk of sudden cardiac death. Pathology of circulation and cardiac surgery. 2018. Vol. 22 (1). S. 16-21. DOI : 10.21688-1681-3472-2018-1-16-21. (In Russian)
[ 17 ] Lazaryc OL, Hryhorieva OA. Lectinhistochemical characteristic of rats’ duodenum reactivity in experiment. Clinical anatomy and operative surgery. 2016; 15(1): 36-38. DOI : 10.24061/1727-0847.15.1.2016.8. (In Ukrainian)
[ 18 ] Lukyanenko NS, Patrica NA, Kens KA. The place of undifferentiated dysplasia of connective tissue in pathology in childhood (literature review). The health of the child. 2015; 2 (61): 80-85. (In Ukrainian)
[ 19 ] Maslovskaya NV, Lollini VA. Undifferentiated dysplasia of connective tissue, and minor heart anomalies as a predictor of development of arrhythmias in patients with ischemic heart disease. Bulletin of Vitebsk state medical University. 2014; 13(3): 68-76. (In Russian)
[ 20 ] Mitchell T, MacDonald JW, Srinouanpranchanh S et al. Evidence of cardiac involvement in the fetal inflammatory response syndrome: disruption of gene networks programming cardiac development in nonhuman primates. Am. J. Obstet. Gynecol. 2018; 218 (4): 438.e1–438.e16. DOI : 10.1016/j.ajog.2018.01.009.
[ 21 ] Mohanta SK, Yin C, Peng L et al. Artery tertiary lymphoid organs contribute to innate and adaptІІІe immune responses in advanced mouse atherosclerosis. Circ. Res. 2014; 114(11): 1772-1787. DOI : 10.1161/CIRCRESAHA.114.301137.
[ 22 ] Mosca M. Mixed connective tissue diseases: new aspects of clinical picture, prognosis and pathogenesis. Isr. Med. Assoc. J. 2014; 16(11)G: 725-726.
[ 23 ] Murphy MO, Cohn DM, Loria AS. Developmental origins of cardiovascular disease: Impact of early life stress in humans and rodents. Neurosci. Biobehav. Rev. 2017: 74(Pt B): 453–465. DOI : 10.1016/j.neubiorev.2016.07.018.
[ 24 ] Nestertzova NS. Features of perinatal development as a basis for the programming of diseases in adults. Collection of scientific works of the Association coserv-gynecologists of Ukraine. 2018; 2 (42): 113-119. (In Russian)
[ 25 ] Oshlyanska AA, Wolf VM. Features of acute respiratory pathology in children with undifferentiated connective tissue dysplasia. Perinatal medicine and Pediatrics. 2017; 1 (69): 115-120. (In Ukrainian)
[ 26 ] Perepelitsa SA. Etiologic and pathogenetic factors of the perinatal development of intrauterine infections in neonates (review). General resuscitation science. 2018; 14 (3): 54-67. DOI : 10.15360/1813-9779-2018-3-54-67. (In Russian)
[ 27 ] Reynolds LP, Borowicz PP, Caton JS et al. Developmental Programming of Fetal Growth and Development. Vet. Clin. North Am. Food Anim. Pract. 2019; 35 (2): 229–247. DOI : 10.1016/j.cvfa.2019.02.006.
[ 28 ] Reznichenko YuG, Lebedynets OM, Voloshin MA. Influence of the pathology of the antenatal period on the morphogenesis and functioning of the cardiovascular system. Perinatal medicine and Pediatrics. 2013. No. 1 (53). S. 82-86. (In Ukrainian)
[ 29 ] Soleyco VA, Kachan VV, Sukhań SS, Chernykh MA. The nature of the lesion of the coronary arteries of patients with chronic coronary heart disease on the background of undifferentiated dysplasia of connective tissue. Bulletin of problems of biology and medicine. 2016; 2-1(128): 112–115. (In Ukrainian)
[ 30 ] Styajkina SN, Knyazef AD, Minakhanov II. Dysplasia of connective tissue in modern clinical practice. Modern innovations. 2016; 5(7):57-64. (In Russian)
[ 31 ] Taboot ET, Karatysh AM. Undifferentiated connective tissue dysplasia. Modern rheumatology. 2009; 3(2): 19-23. DOI : 10.14412/1996-7012-2009-534. (In Russian)
[ 32 ] Tani C, Carli L, Vagnani S, R. Talarico, C. Baldini, M. Mosca et al. The diagnosis and classification of mixed connectІІІe tissue disease // J. Autoimmun. 2014. Vol. 48-49. G. 46-49.
[ 33 ] Tkachenko AK, Romanova ON, Marochkina EM. To the understanding of the term “intrauterine infection”. Journal of Grodno State Medical University. 2017; 1: 103–109
[ 34 ] Ukhov YuS, Sobennikov SS, Coutances SY, Cherenkov AA. Histological interpretation of the severity of dysplasia of connective tissue in clinical practice. Russian medico-biological Bulletin named after academician I. P. Pavlov. 2014; 4: 29-34. (In Russian).
[ 35 ] Voloshyn AN, Chumak AYu. Undifferentiated dysplasia of connective tissue and respiratory diseases in children and adolescents (literature review). The health of the child. 2017; 12 (6) : 720-727.
[ 36 ] Voloshyn NA, Hryhorieva EA, Shcherbakov MS. Intrauterine antigen stimulation as a model to study the morphogenesis of organs. Morphological statements. 2006; 1-2 : 57-59. (In Russian).
[ 37 ] Voloshyn NA, Hryhorieva OA. An experimental model for the development of undifferentiated connective tissue dysplasia syndrome. Pathology. 2009; 6(1) : 39–42.
[ 38 ] Voloshyn NA. Lymphocyte - factor of morphogenesis. Zaporozh. med. Sib. 2005; 2 : 122.
[ 39 ] World health statistics 2018: monitoring health for the SDGs, sustainable development goals. Geneva: World Health Organization; 2018 URL : www.who.int/gho/publications/world_health_statistics/2018/en/
[ 40 ] Zaremba IsH, Cancer NA. Changes of arteries and veins in patients with arterial hypertension with undifferentiated dysplasia of connective tissue. Family medicine. 2017; 1 (69): 69-71. (In Ukrainian).