Ferulic acid in C4 grasses.

Volume 7, Issue 3, June 2022     |     PP. 82-97      |     PDF (325 K)    |     Pub. Date: August 14, 2022
DOI: 10.54647/biology18191    75 Downloads     4966 Views  

Author(s)

Marco Antônio Menezes Neto, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil.
Miguel Pedro Guerra, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina, Florianópolis, SC, P.O. Box 476, 88040-900, Brazil; Graduate Program in Agricultural and Natural Ecosystems, Federal University of Santa Catarina, Curitibanos, Campus Ulysses Gaboardi Road, Km 3, 89520-000, Curitibanos, SC, Brazil

Abstract
In order to identify the phenolic substance that is responsive in a new C4-safranin histochemical test for determination of the photosynthetic pathway in grasses, we developed a fluorescence microscopy aided protocol for extraction and quantification of the target substance, with subsequent mass spectrometry analysis for molecular identification. The results indicate the target phenolic substance in C4 grasses' bundle sheath cells is ferulic acid. In addition, we observed differences in distribution and compartmentalization of ferulic acid depending on the plants’ photosynthetic pathway. In C3 grasses, ferulic acid is restricted to mesophyll cells and concentrated on cell walls, while in C4 grasses it is restricted to bundle sheath cells, and concentrated in the membrane adjacent to radial and tangential walls. Taken together these results indicate that ferulic acid may have an important function related to the C4 photosynthetic pathway in grasses. Such function may be unprecedented and different from any other previously ascribed to this substance.

Keywords
C4 grasses, fluorescence microscopy, ferulic acid.

Cite this paper
Marco Antônio Menezes Neto, Miguel Pedro Guerra, Ferulic acid in C4 grasses. , SCIREA Journal of Biology. Volume 7, Issue 3, June 2022 | PP. 82-97. 10.54647/biology18191

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