Study on Bacterial Diversity and Community Structure in Grape Rhizosphere Soil Based on High-throughput Sequencing

Abstract
In this study, we used the rhizosphere soil of Crimson seedless grape vines with large planting area in Shihezi, Xinjiang as research material, sequenced the bacterial 16S rRNA gene (V4) in different depths of the soil with the grape vines planted for 5, 8, 10, 12 and 15 years by using Illumina MiSeq sequencing platform, and analyzed the diversity and community structure of soil bacteria through bioinformatics related methods. The results showed that 196,690 OTUs were obtained from 45 grape vine rhizosphere soil samples, and the dominant bacterial phyla in the rhizosphere soil were Proteobacteria, Bacteroidetes, Firmicutes, Gemmatimonadetes and Actinobacteria, and the main dominant bacterial genera were Bacteroides, Sphingomonas, Prevotella_9, Lactobacillus and Enterococcus. Redundancy analysis (RDA) showed that soil total potassium and total phosphorus had the greatest influence on bacterial community structure. Correlation analysis showed that all dominant bacterial communities, except for Actinobacteria, were significantly related to soil physicochemical properties. Alpha diversity analysis showed that in 15-25cm deep soil, Shannon index, Chao1 and ACE indices were the highest, indicating that layer had the highest bacterial diversity and community richness; Chao1 and ACE indices of 8-year-old vine group were the highest, indicating that group had the highest bacterial community richness; so, grape vine planting years and soil depth had some influence on the diversity and richness of bacterial communities. Beta diversity and PCoA (principal coordinates analysis) analysis showed that bacterial community structure presented significant difference between different samples. Clustering results also showed that soil depth had some influence on bacterial community structure. Linear discriminant analysis suggested that the 15-year-old vine group had the highest level of biomarkers and classification, and the 15-year-old vine group also had more unique bacterial community (biomarkers) than other age groups, especially in the lower soil layer (25-35cm).

Keywords
grape; rhizosphere soil; high-throughput sequencing; bacterial diversity.

Cite this paper
FengXue, TongLiu, Study on Bacterial Diversity and Community Structure in Grape Rhizosphere Soil Based on High-throughput Sequencing , SCIREA Journal of Biology. Volume 6, Issue 6, December 2021 | PP. 129-153. 10.54647/biology18195

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